Determination Of A Bacteriophage Titer

Determination of a Bacteriophage titre Microbiology Lab promulgate II Title: Determination of a Bacteriophage Titer Purpose: To determine the number of phage particles or plaque-forming units in a suspension of T4 bacteriophage. Materials: 18 24 hour descent culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titre of at least 10,000 phages/ml 5 trypticase soya bean agar (TSA) plates. These should be warmed to 37c before use 5 tubes of aristocratic agar (0.7% agar). Prior to use, melt and hold at 50c in a water bath 5 tubes of 9.

0 ml trypticase soy (TS) broth 1 ml sterile pipettes Pipette support Methods: 1. We began the experiment by marking the plates and place them in lay from 10^5, 10^6, 10^7, then have the uncontaminating dilutions ready in order from 10^-4, 10^-5, 10^-6 2. The pipettes was used and pipette 0.5 ml of the phage suspension to the 10^4 3. The next step was pip...If you want to get a full essay, order it on our website: Orderessay

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